SP6-VEE-OKS-iM | Addgene 58973 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

SP6-VEE-OKS-iM

catalog :

58973

citations: 1

Reference
Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, et al. Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013;13:246-54 pubmed publisher

product information

Catalog Number :

58973

Product Name :

SP6-VEE-OKS-iM

article :

doi	10.1016/j.stem.2013.06.001
id	8771
pubmed_id	23910086

bacterial resistance :

Ampicillin

cloning :

backbone

SP6-VEE-IRES-puro (Addgene plasmid 58971)

backbone_mutation

backbone_origin

Dowdy lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Venezuelan equine encephalitis (VEE) virus RNA replicon

growth notes :

Summary of features: 1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter, nsP1~4 are nonstructural VEE sequences which encode RNA replicases) 7592-8671: human Oct4 8678-8731: T2A peptide sequence 8738-10147: human Klf4 10154-10213: E2A peptide sequence 10223-11176: human Sox2 11195-11805: IRES sequence 11818-13140: human cMyc 13165-13776: IRES sequence 13777-14376: Puromycin resistance gene 14383-14510:VEE 3 UTR and poly A tail 14679-15539: Ampicillin resistance gene 16320-16337: SP6 promoter

growth strain :

RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

origin :

pi :

alt_names

cloning

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

mutation

name

Oct4

shRNA_sequence

size

species

9606

Homo sapiens

tags

alt_names

Kruppel-like factor 4

cloning

entrez_gene

aliases	EZF, GKLF
gene	KLF4
id	9314

genbank_ids

mutation

name

Klf4

shRNA_sequence

size

species

9606

Homo sapiens

tags

alt_names

SRY (sex determining region Y)-box 2

cloning

entrez_gene

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

genbank_ids

mutation

name

Sox2

shRNA_sequence

size

species

9606

Homo sapiens

tags

alt_names

cloning

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

name

cMyc

shRNA_sequence

size

species

9606

Homo sapiens

tags

resistance markers :

1187

tags :

Unknown

terms :

Puromycin