This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
catalog :
58959
citations: 1
product information
Catalog Number :
58959
Product Name :
pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP
article :
| doi | 10.1053/j.gastro.2013.03.013 |
| id | 8795 |
| pubmed_id | 23523670 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pSICO | ||
| backbone_mutation | The loxP sites were deleted and the vector was modified for use with the Gateway Cloning System | ||
| backbone_origin | |||
| backbone_size | 5800 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
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growth notes :
This is a modified version of the plasmid used by Holczbauer et al. The SV40 enhancer/FerH promoter in the original vector has been replaced by the CMV enhancer/chicken beta actin promoter.
growth strain :
lentiviral expression of v-H-Ras and luciferase/EGFP reporter
origin :
30
pi :
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plasmid copy :
This vector was constructed by the Protein Expression Laboratory at the Frederick National Laboratory for Cancer Research. The v-H-Ras gene was obtained from plasmid pBW1423 (Willumsen BM et al. The bovine papillomavirus E5 oncogene can cooperate with ras: identification of p21 amino acids critical for transformation by c-rasH but not v-rasH. Mol Cell Biol. 1991 Dec;11(12):6026-6033).
resistance markers :
| 2042 |
tags :
High Copy
terms :
| Other |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments