This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV HA hRB delta CDK + T821
catalog :
58920
citations: 1
Reference
Narasimha A, Kaulich M, Shapiro G, Choi Y, Sicinski P, Dowdy S. Cyclin D activates the Rb tumor suppressor by mono-phosphorylation. elife. 2014;3: pubmed publisher
product information
Catalog Number :
58920
Product Name :
pCMV HA hRB delta CDK + T821
article :
doi10.7554/eLife.02872
id8750
pubmed_id24876129
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
*CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826
growth strain :
mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored
origin :
37
pi :
alt_names
retinoblastoma tumor suppressor protein
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EBV-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
geneRB1
id5925
genbank_ids
mutationdelta CDK* with T821 restored
nameRb
shRNA_sequence
size2787
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
1187
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA