pET22b RNase A | Addgene 58903 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET22b RNase A

catalog :

58903

citations: 3

Reference
Elnagar M, Ibrahim M, Albert M, M Talal M, Abdelfattah M, El Dabaa E, et al. Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories. AMB Express. 2022;12:137 pubmed publisher
Fuller G, Han T, Freeberg M, Moresco J, Ghanbari Niaki A, Roach N, et al. RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia. elife. 2020;9: pubmed publisher
delCardayre S, Ribo M, Yokel E, Quirk D, Rutter W, Raines R. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995;8:261-73 pubmed

product information

Catalog Number :

58903

Product Name :

pET22b RNase A

article :

doi
id	8788
pubmed_id	7479688

bacterial resistance :

Ampicillin

cloning :

backbone

pET22b(+)

backbone_mutation

backbone_origin

EMD Millipore

backbone_size

5493

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Please note that the pelB sequence is maintained however there are stop codons incorporated at the end of the gene so the His-Tag is not in frame. The pelB is clipped off and the gene encodes 124 amino acid protein that is expressed/purified from inclusion bodies.

growth strain :

Bacterial expression of bovine pancreatic ribonuclease A

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	NcoI
promoter	T7
sequencing_primer_3	T7-Term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	RNS1
gene	RNASE1
id	282340

genbank_ids

NP_001014408

mutation

See Depositor Comments below

name

RNase A

shRNA_sequence

size

500

species

9913

Bos taurus