pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation | Addgene 58830 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

catalog :

58830

citations: 1

Reference
Roy A, Levine T. Multiple pools of phosphatidylinositol 4-phosphate detected using the pleckstrin homology domain of Osh2p. J Biol Chem. 2004;279:44683-9 pubmed

product information

Catalog Number :

58830

Product Name :

pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

article :

doi	10.1074/jbc.M401583200
id	8757
pubmed_id	15271978

bacterial resistance :

Ampicillin

cloning :

backbone

pRS406

backbone_mutation

backbone_origin

Stratagene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

This plasmid contains two copies of Osh2 PH domain. Osh2 PH domain= Proline 256 - Proline 424

growth strain :

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

origin :

pi :

alt_names

Osh2p

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	PHO5
sequencing_primer_3	N/A
sequencing_primer_5	N/A
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YDL019C
gene	OSH2
id	851543

genbank_ids

NM_001180078.1

mutation

changed Lysine 307 to Glutamate and Arginine 309 to Glutamate on both Osh2 copies

name

2 x Osh2 PH domain

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae