pLKO.1 shPIK3CD.v2 puro | Addgene 58707 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO.1 shPIK3CD.v2 puro

catalog :

58707

citations: 1

Reference
Bajikar S, Fuchs C, Roller A, Theis F, Janes K. Parameterizing cell-to-cell regulatory heterogeneities via stochastic transcriptional profiles. Proc Natl Acad Sci U S A. 2014;111:E626-35 pubmed publisher

product information

Catalog Number :

58707

Product Name :

pLKO.1 shPIK3CD.v2 puro

article :

doi	10.1073/pnas.1311647111
id	8726
pubmed_id	24449900

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1

backbone_mutation

backbone_origin

Weinberg Lab (Addgene plasmid 8453)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The primers were generated by the TRC (#TRCN0000033276) Construct mildly knocks down p110d (PIK3CD) protein expression ~2 fold after 3 X 500 uL infection in MCF10A-5E cells. The specificity of this construct has not been confirmed by addback, but a second hairpin against the same target yielded the same phenotype.

growth strain :

Lentiviral shRNA vector for knockdown of human PIK3CD

origin :

pi :

sapienstags
p110DgenePIK
3CDid5293d><
/tr>genbank_ids

NM_005026

>mutationr>nameshPIK3CDr>shRNA_sequenceCCGGGA
CCCAGAAGTGAACGACTTTCTGCAGAAAGTTCACTTCTG
GGTCTTTTTGsized>speciesble>r>

9606

Homo

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases

APDS, IMD14, IMD14A, IMD14B, P110DELTA, PI3K, ROCHIS,

resistance markers :

1585

tags :

High Copy

terms :

Puromycin