pLKO.1 shTGFBR3 puro | Addgene 58696 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLKO.1 shTGFBR3 puro

catalog :

58696

citations: 3

Reference
Hsu Y, Yin Y, Tsai K, Jian C, Liang Z, Hsu C, et al. TGFBR3 supports anoikis through suppressing ATF4 signaling. J Cell Sci. 2022;135: pubmed publisher
Bajikar S, Wang C, Borten M, Pereira E, Atkins K, Janes K. Tumor-Suppressor Inactivation of GDF11 Occurs by Precursor Sequestration in Triple-Negative Breast Cancer. Dev Cell. 2017;43:418-435.e13 pubmed publisher
Wang C, Bajikar S, Jamal L, Atkins K, Janes K. A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Nat Cell Biol. 2014;16:345-56 pubmed publisher

product information

Catalog Number :

58696

Product Name :

pLKO.1 shTGFBR3 puro

article :

doi	10.1038/ncb2930
id	8689
pubmed_id	24658685

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1

backbone_mutation

backbone_origin

Weinberg Lab (Addgene plasmid 8453)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

This construct was obtained from the TRC (#TRCN0000033430) and potently knocks down TGFBR3 expression after 3 x 500 microL infection in MCF10A cells.

growth strain :

Lentiviral shRNA vector for knockdown of human TGFBR3

origin :

pi :

sapienstags
betaglycangened>TGFBR3id70
49<
/td>genbank_idsable>NM_001195683ble>mutation
nameshTGFBR3
shRNA_sequenced>ccggCCAAGCATGAAGGAACCAAATctcgagATTTGG
TTCCTTCATGCTTGGtttttg
sizespecies<
/td>

9
606

Homo

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases

BGCAN,

resistance markers :

1585

tags :

High Copy

terms :

Puromycin