This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe RFP1-KRT5 hygro
catalog :
58493
citations: 3
Reference
Nanes B, Bhatt K, Azarova E, Rajendran D, Isogai T, Dean K, et al. Keratin isoform shifts modulate motility signals during wound healing. bioRxiv. 2023;: pubmed publisher
McGinn O, Ward A, Fettig L, Riley D, Ivie J, Paul K, et al. Cytokeratin 5 alters β-catenin dynamics in breast cancer cells. Oncogene. 2020;39:2478-2492 pubmed publisher
Wang C, Bajikar S, Jamal L, Atkins K, Janes K. A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. Nat Cell Biol. 2014;16:345-56 pubmed publisher
product information
Catalog Number :
58493
Product Name :
pBabe RFP1-KRT5 hygro
article :
doi10.1038/ncb2930
id8689
pubmed_id24658685
bacterial resistance :
Ampicillin
cloning :
backbonepBabe N-terminal RFP hygro
backbone_mutationThis construct is a variant of pBabe hygro that expresses mRFP1 as an N-terminal fusion. A new XhoI site was inserted immediately upstream of the BamHI site by PCR during cloning. TO EXPRESS AN N-mRFP1 FUSION, GENES MUST HAVE A BAMHI- OR XHO1-COMPATIBLE 5' STICKY END.
backbone_origin
backbone_size6265
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pBabe RFP1_KRT5 hygro was constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe N-terminal RFP hygro. The PCR product was gel purified, digested with BamHI and EcoRI, and then ligated into pBabe N-terminal RFP hygro, which had been digested with BamHI and EcoRI and then dephosphorylated.
growth strain :
mammalian expression of mRFP1-tagged human KRT5
origin :
37
pi :
alt_names
keratin 5
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3pBABE 3'
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCK5, DDD, DDD1, EBS1, EBS2, EBS2A, EBS2B, EBS2C, EBS2D, EBS2E, EBS2F, K5, KRT5A
geneKRT5
id3852
genbank_ids
NM_000424
mutation
nameKRT5
shRNA_sequence
size1783
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmRFP1
resistance markers :
1585
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA