This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmCherry-C1 R62D actin-3XNLS P2A mCherry
catalog :
58477
citations: 3
Reference
Mishra S, Levy D. Nuclear F-actin and Lamin A antagonistically modulate nuclear shape. J Cell Sci. 2022;135: pubmed publisher
Titelbaum M, Brant B, Baumel D, Burstein Willensky A, Perez S, Barsheshet Y, et al. Ezh2 harnesses the intranuclear actin cytoskeleton to remodel chromatin in differentiating Th cells. iScience. 2021;24:103093 pubmed publisher
Belin B, Lee T, Mullins R. DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected]. elife. 2015;4:e07735 pubmed publisher
product information
Catalog Number :
58477
Product Name :
pmCherry-C1 R62D actin-3XNLS P2A mCherry
article :
doi10.7554/eLife.07735
id15847
pubmed_id26287480
bacterial resistance :
Kanamycin
cloning :
backbonemCherry-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3mCherry Reverse
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
NM_001101.3
mutationChanged Arginine 62 to Aspartic Acid
namebeta actin
shRNA_sequence
size1125
species
9606
Homo sapiens
tags
alt_names
nuclear localization signal
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3mCherry Reverse
sequencing_primer_5actin Forward
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name3XNLS
shRNA_sequence
size72
species
32644
Other
SV40 virus
tags
alt_names
2A Peptide Derived from Porcine Teschovirus-1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3mCherry Reverse
sequencing_primer_5actin Forward
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameP2A cleavage site
shRNA_sequence
size
species
32644
Other
Porcine Teschovirus-1
tags
locationC terminal on insert
tagmCherry
plasmid copy :
P2A peptide and 3X NLS (with stop codons) were inserted into the pmCherry-C1 backbone via GenScript's custom cloning services. Human actin was cloned from cDNA using Gibson cloning into an NLS-P2A-mCherry backbone.
resistance markers :
2164
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA