This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmCherry-C1 actin-3XNLS P2A mCherry
catalog :
58475
citations: 2
Reference
Mishra S, Levy D. Nuclear F-actin and Lamin A antagonistically modulate nuclear shape. J Cell Sci. 2022;135: pubmed publisher
Belin B, Lee T, Mullins R. DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair. [corrected]. elife. 2015;4:e07735 pubmed publisher
product information
Catalog Number :
58475
Product Name :
pmCherry-C1 actin-3XNLS P2A mCherry
article :
doi10.7554/eLife.07735
id15847
pubmed_id26287480
bacterial resistance :
Kanamycin
cloning :
backbonemCherry-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3mCherry Reverse
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
NM_001101.3
mutation
namebeta actin
shRNA_sequence
size1125
species
9606
Homo sapiens
tags
alt_names
nuclear localization signal
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3mCherry Reverse
sequencing_primer_5actin Forward
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name3XNLS
shRNA_sequence
size72
species
32644
Other
SV40 virus
tags
alt_names
2A Peptide Derived from Porcine Teschovirus-1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3mCherry Reverse
sequencing_primer_5actin Forward
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameP2A cleavage site
shRNA_sequence
size
species
32644
Other
Porcine Teschovirus-1
tags
locationC terminal on insert
tagmCherry
plasmid copy :
P2A peptide and 3X NLS (with stop codons) were inserted into the pmCherry-C1 backbone via GenScript's custom cloning services. Human actin was cloned from cDNA using Gibson cloning into an NLS-P2A-mCherry backbone.
resistance markers :
2164
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA