This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-C1 F-tractin-EGFP
catalog :
58473
citations: 18
Reference
Weems A, Welf E, Driscoll M, Zhou F, Mazloom Farsibaf H, Chang B, et al. Blebs promote cell survival by assembling oncogenic signalling hubs. Nature. 2023;615:517-525 pubmed publisher
Kokate S, Ciuba K, Tran V, Kumari R, Tojkander S, Engel U, et al. Caldesmon controls stress fiber force-balance through dynamic cross-linking of myosin II and actin-tropomyosin filaments. Nat Commun. 2022;13:6032 pubmed publisher
Maity D, Bera K, Li Y, Ge Z, Ni Q, Konstantopoulos K, et al. Extracellular Hydraulic Resistance Enhances Cell Migration. Adv Sci (Weinh). 2022;9:e2200927 pubmed publisher
Tang W, Liu Y, Yeh C, Lu C, Tu C, Lin Y, et al. Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy. Commun Biol. 2022;5:879 pubmed publisher
Herron J, Hu S, Watanabe T, Nogueira A, Liu B, Kern M, et al. Actin nano-architecture of phagocytic podosomes. Nat Commun. 2022;13:4363 pubmed publisher
Vosatka K, Lavenus S, Logue J. A novel Fiji/ImageJ plugin for the rapid analysis of blebbing cells. PLoS ONE. 2022;17:e0267740 pubmed publisher
Choudhury M, Li Y, Mistriotis P, Vasconcelos A, DIXON E, Yang J, et al. Kidney epithelial cells are active mechano-biological fluid pumps. Nat Commun. 2022;13:2317 pubmed publisher
Pathni A, Ozcelikkale A, Rey Suárez I, Li L, Davis S, Rogers N, et al. Cytotoxic T Lymphocyte Activation Signals Modulate Cytoskeletal Dynamics and Mechanical Force Generation. Front Immunol. 2022;13:779888 pubmed publisher
Alfonzo Méndez M, Sochacki K, Strub M, Taraska J. Dual clathrin and integrin signaling systems regulate growth factor receptor activation. Nat Commun. 2022;13:905 pubmed publisher
Cheah J, Jacobs K, Lai T, Caballelo R, Yee J, Ueda S, et al. Spatial proximity of proteins surrounding zyxin under force-bearing conditions. Mol Biol Cell. 2021;32:1221-1228 pubmed publisher
Moore A, Coscia S, Simpson C, Ortega F, Wait E, Heddleston J, et al. Actin cables and comet tails organize mitochondrial networks in mitosis. Nature. 2021;591:659-664 pubmed publisher
Wagh K, Wheatley B, Traver M, Hussain I, Schaefer B, Upadhyaya A. Bcl10 is associated with actin dynamics at the T cell immune synapse. Cell Immunol. 2020;356:104161 pubmed publisher
Nelsen E, Hobson C, Kern M, Hsiao J, O Brien Iii E, Watanabe T, et al. Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies. Sci Rep. 2020;10:8133 pubmed publisher
Valenzuela J, Perez F. Localized Intercellular Transfer of Ephrin-As by Trans-endocytosis Enables Long-Term Signaling. Dev Cell. 2019;: pubmed publisher
Cheah J, Jacobs K, Heinrich V, Lo S, Yamada S. Force-induced recruitment of cten along keratin network in epithelial cells. Proc Natl Acad Sci U S A. 2019;116:19799-19801 pubmed publisher
Guo M, Chandris P, Giannini J, Trexler A, Fischer R, Chen J, et al. Single-shot super-resolution total internal reflection fluorescence microscopy. Nat Methods. 2018;15:425-428 pubmed publisher
Spiliotis E, Karasmanis E, Dolat L. Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cells. Methods Cell Biol. 2016;136:243-68 pubmed publisher
Belin B, Goins L, Mullins R. Comparative analysis of tools for live cell imaging of actin network architecture. Bioarchitecture. 2014;4:189-202 pubmed publisher
product information
Catalog Number :
58473
Product Name :
pEGFP-C1 F-tractin-EGFP
article :
doi10.1080/19490992.2014.1047714
id15846
pubmed_id26317264
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoter
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFP Reverse
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneItpka
id81677
genbank_ids
NM_031045.2
mutation
nameF-tractin
shRNA_sequence
size727
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagEGFP
plasmid copy :
F-tractin was inserted into the pEGFP-C1 backbone via GenScript's custom cloning services.
resistance markers :
2164
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA