This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-C1 RPEL1-EGFP-3XNLS
catalog :
58469
citations: 2
Reference
Hurst V, Challa K, Shimada K, Gasser S. Cytoskeleton integrity influences XRCC1 and PCNA dynamics at DNA damage. Mol Biol Cell. 2021;32:br6 pubmed publisher
Belin B, Cimini B, Blackburn E, Mullins R. Visualization of actin filaments and monomers in somatic cell nuclei. Mol Biol Cell. 2013;24:982-94 pubmed publisher
product information
Catalog Number :
58469
Product Name :
pEGFP-C1 RPEL1-EGFP-3XNLS
article :
doi10.1091/mbc.E12-09-0685
id8685
pubmed_id23447706
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4686
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5NheI
promoterCMV
sequencing_primer_3EGFP Reverse
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAMKL, Bsac, Mal, Mkl1, Mrtf-A
geneMrtfa
id223701
genbank_ids
AF532597
mutation
nameMRTF-A RPEL1
shRNA_sequence
size99
species
10090
Mus musculus
100
Synthetic
tags
locationC terminal on insert
tagEGFP
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SV40 polyA Reverse
sequencing_primer_5EGFP Forward
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name3X NLS with 3X stop codons
shRNA_sequence
size72
species
32644
Other
SV40 virus
tags
locationN terminal on insert
tagEGFP
plasmid copy :
3X NLS (with stop codons) was cloned by insertion of annealed primers with flanking restriction sites.
resistance markers :
2164
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA