pAAV/D374Y-hPCSK9 | Addgene 58379 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV/D374Y-hPCSK9

catalog :

58379

citations: 2

Reference
Jarrett K, Lee C, De Giorgi M, Hurley A, Gillard B, Doerfler A, et al. Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research. Arterioscler Thromb Vasc Biol. 2018;: pubmed publisher
Bjørklund M, Hollensen A, Hagensen M, Dagnaes Hansen F, Christoffersen C, Mikkelsen J, et al. Induction of atherosclerosis in mice and hamsters without germline genetic engineering. Circ Res. 2014;114:1684-9 pubmed publisher

product information

Catalog Number :

58379

Product Name :

pAAV/D374Y-hPCSK9

article :

doi	10.1161/CIRCRESAHA.114.302937
id	8668
pubmed_id	24677271

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV/mU6.siRNA.shuttle

backbone_mutation

mU6.siRNA cassette removed

backbone_origin

Applied Viromics, Fremont, CA, USA

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth notes :

Insert contains G670E and V474I, which are known SNPs in the human PCSK9 gene,

growth strain :

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

origin :

pi :

alt_names

PCSK9

NARC1

cloning

clone_method	Restriction Enzyme
cloning_site_3	FseI
cloning_site_5	AgeI
promoter	HCRApoE/hAAT
sequencing_primer_3	na
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9
gene	PCSK9
id	255738

genbank_ids

mutation

D374Y

name

proprotein convertase subtilisin/kexin type 9

shRNA_sequence

size

2078

species

9606

Homo sapiens