pCAG-HA-rat Nrxn2beta AS4(-) | Addgene 58268 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCAG-HA-rat Nrxn2beta AS4(-)

catalog :

58268

citations: 1

Reference
Taniguchi H, Gollan L, Scholl F, Mahadomrongkul V, Dobler E, Limthong N, et al. Silencing of neuroligin function by postsynaptic neurexins. J Neurosci. 2007;27:2815-24 pubmed

product information

Catalog Number :

58268

Product Name :

pCAG-HA-rat Nrxn2beta AS4(-)

article :

doi	10.1523/JNEUROSCI.0032-07.2007
id	8632
pubmed_id	17360903

bacterial resistance :

Ampicillin

cloning :

backbone

pCAGGS

backbone_mutation

backbone_origin

backbone_size

4870

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

See map & sequence file for insert-releasing enzymes (e.g. NheI / Bsu36I). Insert can also be released by single digest using XhoI. The rat neurexin 2 beta encoded by this plasmid lacks alternatively spliced segment 4 (AS4). Note: HA-epitope tag was introduced at the mature N-terminus.

growth strain :

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

origin :

pi :

alt_names

Neurexin2beta AS4(-)

Neurexin2beta Delta4

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CAG
sequencing_primer_3	Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
sequencing_primer_5	pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Nrxn2
id	116595

genbank_ids

M96377

mutation

name

Nrxn2beta AS4(-)

shRNA_sequence

size

1347

species

10116

Rattus norvegicus