pCAG-HA-rat Nrxn1alpha | Addgene 58266 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCAG-HA-rat Nrxn1alpha

catalog :

58266

citations: 4

Reference
de Arce K, Ribic A, Chowdhury D, Watters K, Thompson G, Sanganahalli B, et al. Concerted roles of LRRTM1 and SynCAM 1 in organizing prefrontal cortex synapses and cognitive functions. Nat Commun. 2023;14:459 pubmed publisher
William N, Reissner C, SARGENT R, Darlington T, Diblasi E, Li Q, et al. Neurexin 1 variants as risk factors for suicide death. Mol Psychiatry. 2021;: pubmed publisher
Meng X, McGraw C, Wang W, Jing J, Yeh S, Wang L, et al. Neurexophilin4 is a selectively expressed α-neurexin ligand that modulates specific cerebellar synapses and motor functions. elife. 2019;8: pubmed publisher
Taniguchi H, Gollan L, Scholl F, Mahadomrongkul V, Dobler E, Limthong N, et al. Silencing of neuroligin function by postsynaptic neurexins. J Neurosci. 2007;27:2815-24 pubmed

product information

Catalog Number :

58266

Product Name :

pCAG-HA-rat Nrxn1alpha

article :

doi	10.1523/JNEUROSCI.0032-07.2007
id	8632
pubmed_id	17360903

bacterial resistance :

Ampicillin

cloning :

backbone

pCAGGS

backbone_mutation

backbone_origin

backbone_size

4869

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI). Insert can also be released without HA-tag (e.g. by SpeI / XmaI digest). The rat neurexin 1 alpha encoded by this plasmid lacks alternatively spliced segment 4 (AS4), and contains the regions homologous to alternatively spliced segment 3 and 5 (AS3 and AS5, respectively) of mouse neurexin 1 alpha (compare Schreiner et al., Neuron, 2014 [DOI: 10.1016/j.neuron.2014.09.011]). Note: HA-epitope tag was introduced at the mature N-terminus.

growth strain :

Expression plasmid for HA-tagged rat Neurexin 1alpha

origin :

pi :

alt_names

neurexin 1alpha

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CAG
sequencing_primer_3	Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
sequencing_primer_5	pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Nrxn1b
gene	Nrxn1
id	60391

genbank_ids

NM_021767

mutation

see comments

name

Nrxn1alpha

shRNA_sequence

size

4434

species

10116

Rattus norvegicus