This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMIR-DsRed-IRES-His-Halo-Tev-Keap1
catalog :
58240
citations: 4
Reference
Pei J, Xiao Y, Liu X, Hu W, Sobh A, Yuan Y, et al. Piperlongumine conjugates induce targeted protein degradation. Cell Chem Biol. 2023;30:203-213.e17 pubmed publisher
Zhang W, Cheng C, Sha Z, Chen C, Yu C, Lv N, et al. Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress. Free Radic Biol Med. 2021;168:247-257 pubmed publisher
Parvez S, Long M, Lin H, Zhao Y, Haegele J, Pham V, et al. T-REX on-demand redox targeting in live cells. Nat Protoc. 2016;11:2328-2356 pubmed publisher
Fang X, Fu Y, Long M, Haegele J, Ge E, Parvez S, et al. Temporally controlled targeting of 4-hydroxynonenal to specific proteins in living cells. J Am Chem Soc. 2013;135:14496-9 pubmed publisher
product information
Catalog Number :
58240
Product Name :
pMIR-DsRed-IRES-His-Halo-Tev-Keap1
article :
doi10.1021/ja405400k
id8609
pubmed_id24015839
bacterial resistance :
Ampicillin
cloning :
backbonepMIR
backbone_mutation
backbone_originAddgene
backbone_size6470
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Yen, H-C.S., Xu, Q., Chou, D.M., Zhao, Z., and Elledge, S.J. (2008). Global protein stability profiling in mammalian cells. Science 322, 918 923 Aye, Y.; Brignole, E. J.; Long, M. J. C.; Chitturulu, J.; Drennan, C. L.; Asturias, F. J.; Stubbe J. Chem. Biol. 2012, 19, 799-805
growth strain :
expresses halo tagged human Keap1 protein
origin :
37
pi :
alt_names
Keap1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3CAGGAAACAGCTATGAC
sequencing_primer_5TGGCTCTCCTCAAGCGTATT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesINrf2, KLHL19
geneKEAP1
id9817
genbank_ids
NM_203500.1
NM_012289.3
mutation
nameKelch like ECH associated protein 1
shRNA_sequence
size2859
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis-Halo-Tev
resistance markers :
2000
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA