This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV CEPIA4mt
catalog :
58220
citations: 2
Reference
Roberts R, Vervliet T, Bultynck G, Parys J, Hallett M. EPIC3, a novel Ca2+ indicator located at the cell cortex and in microridges, detects high Ca2+ subdomains during Ca2+ influx and phagocytosis. Cell Calcium. 2020;92:102291 pubmed publisher
Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M. Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA. Nat Commun. 2014;5:4153 pubmed publisher
product information
Catalog Number :
58220
Product Name :
pCMV CEPIA4mt
article :
doi10.1038/ncomms5153
id8647
pubmed_id24923787
bacterial resistance :
Ampicillin
cloning :
backbonepCMV/myc/mito
backbone_mutationDuplicate mitochondria targeting sequence.
backbone_originInvitrogen
backbone_size4799
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)
origin :
37
pi :
alt_names
green fluorescent calcium-measuring organelle-entrapped protein indicator for the mitochondria (low affinity variant)
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5None
promoterCMV
sequencing_primer_3tagaaggcacagtcgagg-
sequencing_primer_5cgcaaatgggcggtaggcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationGCaMP2 N263Y E282V E334D M339L F395W D436E
nameCEPIA4mt
shRNA_sequence
size1605
species
100
Synthetic
tags
locationC terminal on insert
tagmyc
resistance markers :
1996
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA