This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV CEPIA3mt
catalog :
58219
citations: 17
Reference
Eom Y, Kim S, Kim Y, Lee S. Mitochondrial Calcium Waves by Electrical Stimulation in Cultured Hippocampal Neurons. Mol Neurobiol. 2023;: pubmed publisher
Fernandez Garcia E, Paudel U, Noji M, Bowman C, Rustgi A, Pitarresi J, et al. The mitochondrial Ca2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferation. Front Cell Dev Biol. 2023;11:1082213 pubmed publisher
Jost P, Klein F, Brand B, Wahl V, Wyatt A, Yildiz D, et al. Acute Downregulation but Not Genetic Ablation of Murine MCU Impairs Suppressive Capacity of Regulatory CD4 T Cells. Int J Mol Sci. 2023;24: pubmed publisher
Ni Y, Liu J, Zeng L, Yang Y, Liu L, Yao M, et al. Natural product manoalide promotes EGFR-TKI sensitivity of lung cancer cells by KRAS-ERK pathway and mitochondrial Ca2+ overload-induced ferroptosis. Front Pharmacol. 2022;13:1109822 pubmed publisher
Katona M, Bart xf3 k x, Nichtova Z, Csord xe1 s G, Berezhnaya E, Weaver D, et al. Capture at the ER-mitochondrial contacts licenses IP3 receptors to stimulate local Ca2+ transfer and oxidative metabolism. Nat Commun. 2022;13:6779 pubmed publisher
Li X, Kordsmeier J, Nookaew I, Kim H, Xiong J. Piezo1 stimulates mitochondrial function via cAMP signaling. FASEB J. 2022;36:e22519 pubmed publisher
Wolf C, Pouya A, Bitar S, Pfeiffer A, Bueno D, Rojas Charry L, et al. GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeleton. Commun Biol. 2022;5:541 pubmed publisher
Roberts R, Vervliet T, Bultynck G, Parys J, Hallett M. EPIC3, a novel Ca2+ indicator located at the cell cortex and in microridges, detects high Ca2+ subdomains during Ca2+ influx and phagocytosis. Cell Calcium. 2020;92:102291 pubmed publisher
Debattisti V, Horn A, Singh R, Seifert E, Hogarth M, Mazala D, et al. Dysregulation of Mitochondrial Ca2+ Uptake and Sarcolemma Repair Underlie Muscle Weakness and Wasting in Patients and Mice Lacking MICU1. Cell Rep. 2019;29:1274-1286.e6 pubmed publisher
Olivas Aguirre M, Torres López L, Valle Reyes J, Hernandez Cruz A, Pottosin I, Dobrovinskaya O. Cannabidiol directly targets mitochondria and disturbs calcium homeostasis in acute lymphoblastic leukemia. Cell Death Dis. 2019;10:779 pubmed publisher
Safiulina D, Kuum M, Choubey V, Gogichaishvili N, Liiv J, Hickey M, et al. Miro proteins prime mitochondria for Parkin translocation and mitophagy. EMBO J. 2019;38: pubmed publisher
de la Fuente S, Lambert J, Nichtova Z, Fernandez Sanz C, Elrod J, Sheu S, et al. Spatial Separation of Mitochondrial Calcium Uptake and Extrusion for Energy-Efficient Mitochondrial Calcium Signaling in the Heart. Cell Rep. 2018;24:3099-3107.e4 pubmed publisher
Böhler P, Stuhldreier F, Anand R, Kondadi A, Schlütermann D, Berleth N, et al. The mycotoxin phomoxanthone A disturbs the form and function of the inner mitochondrial membrane. Cell Death Dis. 2018;9:286 pubmed publisher
Cho B, Cho H, Jo Y, Kim H, Song M, Moon C, et al. Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division. Nat Commun. 2017;8:15754 pubmed publisher
Vervloessem T, Ivanova H, Luyten T, Parys J, Bultynck G. The selective Bcl-2 inhibitor venetoclax, a BH3 mimetic, does not dysregulate intracellular Ca2+ signaling. Biochim Biophys Acta Mol Cell Res. 2017;1864:968-976 pubmed publisher
Eraso Pichot A, Larramona Arcas R, Vicario Orri E, Villalonga R, Pardo L, Galea E, et al. CREB decreases astrocytic excitability by modifying subcellular calcium fluxes via the sigma-1 receptor. Cell Mol Life Sci. 2017;74:937-950 pubmed publisher
Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M. Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA. Nat Commun. 2014;5:4153 pubmed publisher
product information
Catalog Number :
58219
Product Name :
pCMV CEPIA3mt
article :
doi10.1038/ncomms5153
id8647
pubmed_id24923787
bacterial resistance :
Ampicillin
cloning :
backbonepCMV/myc/mito
backbone_mutationDuplicate mitochondria targeting sequence.
backbone_originInvitrogen
backbone_size4799
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)
origin :
37
pi :
alt_names
green fluorescent calcium-measuring organelle-entrapped protein indicator for the mitochondria (moderately low affinity variant)
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5None
promoterCMV
sequencing_primer_3tagaaggcacagtcgagg
sequencing_primer_5cgcaaatgggcggtaggcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationGCaMP2 N263Y E282V E334D M339L
nameCEPIA3mt
shRNA_sequence
size1605
species
100
Synthetic
tags
locationC terminal on insert
tagmyc
resistance markers :
1996
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA