This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV CEPIA2mt
catalog :
58218
citations: 18
Reference
Clement D, Szabo E, Krokeide S, Wiiger M, Vincenti M, Palacios D, et al. The Lysosomal Calcium Channel TRPML1 Maintains Mitochondrial Fitness in NK Cells through Interorganelle Cross-Talk. J Immunol. 2023;211:1348-1358 pubmed publisher
Fernandez Garcia E, Paudel U, Noji M, Bowman C, Rustgi A, Pitarresi J, et al. The mitochondrial Ca2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferation. Front Cell Dev Biol. 2023;11:1082213 pubmed publisher
Tanwar J, Ahuja K, Sharma A, Sehgal P, Ranjan G, Sultan F, et al. Mitochondrial calcium signaling mediated transcriptional regulation of keratin filaments is a critical determinant of melanogenesis. bioRxiv. 2023;: pubmed publisher
Sutandy F, G xf6 xdf ner I, Tascher G, M xfc nch C. A cytosolic surveillance mechanism activates the mitochondrial UPR. Nature. 2023;618:849-854 pubmed publisher
Dhoundiyal A, Goeschl V, Boehm S, Kubista H, Hotka M. Glycerol-3-phosphate shuttle is a backup system securing metabolic flexibility in neurons. J Neurosci. 2022;42:7339-54 pubmed publisher
Xue Y, Morris J, Yang K, Fu Z, Zhu X, Johnson F, et al. SMARCA4/2 loss inhibits chemotherapy-induced apoptosis by restricting IP3R3-mediated Ca2+ flux to mitochondria. Nat Commun. 2021;12:5404 pubmed publisher
Stavsky A, Stoler O, Kostić M, Katoshevsky T, Assali E, Savić I, et al. Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation. Commun Biol. 2021;4:666 pubmed publisher
Goldberg J, Currais A, Ates G, Huang L, Shokhirev M, Maher P, et al. Targeting of intracellular Ca2+ stores as a therapeutic strategy against age-related neurotoxicities. NPJ Aging Mech Dis. 2020;6:10 pubmed publisher
Byts N, Sharma S, Laurila J, Paudel P, Miinalainen I, Ronkainen V, et al. Transmembrane Prolyl 4-Hydroxylase is a Novel Regulator of Calcium Signaling in Astrocytes. Eneuro. 2021;8: pubmed publisher
Roberts R, Vervliet T, Bultynck G, Parys J, Hallett M. EPIC3, a novel Ca2+ indicator located at the cell cortex and in microridges, detects high Ca2+ subdomains during Ca2+ influx and phagocytosis. Cell Calcium. 2020;92:102291 pubmed publisher
Hotka M, Cagalinec M, Hilber K, Hool L, Boehm S, Kubista H. L-type Ca2+ channel-mediated Ca2+ influx adjusts neuronal mitochondrial function to physiological and pathophysiological conditions. Sci Signal. 2020;13: pubmed publisher
Nardin C, Peres C, Mazzarda F, Ziraldo G, Salvatore A, Mammano F. Photosensitizer Activation Drives Apoptosis by Interorganellar Ca2+ Transfer and Superoxide Production in Bystander Cancer Cells. Cells. 2019;8: pubmed publisher
Kostić M, Katoshevski T, Sekler I. Allosteric Regulation of NCLX by Mitochondrial Membrane Potential Links the Metabolic State and Ca2+ Signaling in Mitochondria. Cell Rep. 2018;25:3465-3475.e4 pubmed publisher
Courjaret R, Dib M, Machaca K. Spatially restricted subcellular Ca2+ signaling downstream of store-operated calcium entry encoded by a cortical tunneling mechanism. Sci Rep. 2018;8:11214 pubmed publisher
Weatherly L, Nelson A, Shim J, Riitano A, Gerson E, Hart A, et al. Antimicrobial agent triclosan disrupts mitochondrial structure, revealed by super-resolution microscopy, and inhibits mast cell signaling via calcium modulation. Toxicol Appl Pharmacol. 2018;349:39-54 pubmed publisher
Prudent J, Popgeorgiev N, Gadet R, Deygas M, Rimokh R, Gillet G. Mitochondrial Ca2+ uptake controls actin cytoskeleton dynamics during cell migration. Sci Rep. 2016;6:36570 pubmed publisher
Yun B, Lee H, Ewing H, Gelb M, Leslie C. Off-target effect of the cPLA2? inhibitor pyrrophenone: Inhibition of calcium release from the endoplasmic reticulum. Biochem Biophys Res Commun. 2016;479:61-6 pubmed publisher
Suzuki J, Kanemaru K, Ishii K, Ohkura M, Okubo Y, Iino M. Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA. Nat Commun. 2014;5:4153 pubmed publisher
product information
Catalog Number :
58218
Product Name :
pCMV CEPIA2mt
article :
doi10.1038/ncomms5153
id8647
pubmed_id24923787
bacterial resistance :
Ampicillin
cloning :
backbonepCMV/myc/mito
backbone_mutationDuplicate mitochondria targeting sequence.
backbone_originInvitrogen
backbone_size4799
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Green fluorescent indicator for calcium imaging in the mitochondria
origin :
37
pi :
alt_names
green fluorescent calcium-measuring organelle-entrapped protein indicator for the mitochondria
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5unknown
promoterCMV
sequencing_primer_3tagaaggcacagtcgagg
sequencing_primer_5cgcaaatgggcggtaggcgtg
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationGCaMP2 N263Y E282V M339L
nameCEPIA2mt
shRNA_sequence
size1605
species
100
Synthetic
tags
locationC terminal on insert
tagmyc
resistance markers :
1996
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA