This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Dendra2-Vinculin-N-21
catalog :
57749
citations: 3
Reference
Xu Z, Rui Y, Hagan J, Kim D. Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction Endonucleases. Bio Protoc. 2018;8: pubmed publisher
Xu Z, Rui Y, Balzeau J, Menezes M, Niu A, Hagan J, et al. Highly efficient one-step scarless protein tagging by type IIS restriction endonuclease-mediated precision cloning. Biochem Biophys Res Commun. 2017;490:8-16 pubmed publisher
Shtengel G, Galbraith J, Galbraith C, Lippincott Schwartz J, Gillette J, Manley S, et al. Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Proc Natl Acad Sci U S A. 2009;106:3125-30 pubmed publisher
product information
Catalog Number :
57749
Product Name :
Dendra2-Vinculin-N-21
article :
doi10.1073/pnas.0813131106
id8523
pubmed_id19202073
bacterial resistance :
Kanamycin
cloning :
backboneDendra2-N1
backbone_mutation
backbone_origin
backbone_size4750
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
. Excitation = 490 / 553; Emission = 507 / 573
growth strain :
Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573
origin :
37
pi :
alt_names
NM_003373.3
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMD1W, CMH15, HEL114, MV, MVCL, VINC
geneVCL
id7414
genbank_ids
mutation
nameNM_003373.3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagDendra2
resistance markers :
1976
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA