This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
G protein alpha-s(alpha3beta5/G226A/A366S)
catalog :
55797
citations: 1
Reference
Berlot C. A highly effective dominant negative alpha s construct containing mutations that affect distinct functions inhibits multiple Gs-coupled receptor signaling pathways. J Biol Chem. 2002;277:21080-5 pubmed
product information
Catalog Number :
55797
Product Name :
G protein alpha-s(alpha3beta5/G226A/A366S)
article :
doi10.1074/jbc.M201330200
id8568
pubmed_id11927592
bacterial resistance :
Ampicillin
cloning :
backbonepcDNAI/Amp
backbone_mutation
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
origin :
37
pi :
alt_names
Gnas
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6
geneGnas
id24896
genbank_ids
mutationN271K, K274D, R280K, T284D, I285T, G226A, A366S in alpha-s sequence
namealpha-s (alpha3beta5/G226A/A366S)
shRNA_sequence
size1586
species
10116
Rattus norvegicus
tags
location
taginternal EE epitope (residues 189-194 in alpha-s sequence are EYMPTE)
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA