This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Dyn1(K44A)-mRFP
catalog :
55795
citations: 6
Reference
Piacentino M, Hutchins E, Andrews C, Bronner M. Temporal changes in plasma membrane lipid content induce endocytosis to regulate developmental epithelial-to-mesenchymal transition. Proc Natl Acad Sci U S A. 2022;119:e2212879119 pubmed publisher
Shin W, Zucker B, Kundu N, Lee S, Shi B, Chan C, et al. Molecular mechanics underlying flat-to-round membrane budding in live secretory cells. Nat Commun. 2022;13:3697 pubmed publisher
Li B, He X, Dong Z, Xuan K, Sun W, Gao L, et al. Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca2+ channel internalization in mesenchymal stem cells. Bone Res. 2020;8:19 pubmed publisher
Zahavi E, Steinberg N, Altman T, Chein M, Joshi Y, Gradus Pery T, et al. The receptor tyrosine kinase TrkB signals without dimerization at the plasma membrane. Sci Signal. 2018;11: pubmed publisher
Shin W, Ge L, Arpino G, Villarreal S, HAMID E, Liu H, et al. Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis. Cell. 2018;173:934-945.e12 pubmed publisher
Hynes T, Mervine S, Yost E, Sabo J, Berlot C. Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. J Biol Chem. 2004;279:44101-12 pubmed
product information
Catalog Number :
55795
Product Name :
Dyn1(K44A)-mRFP
article :
doi10.1074/jbc.M405151200
id8567
pubmed_id15297467
bacterial resistance :
Kanamycin
cloning :
backboneDerived from pEGFP-N1
backbone_mutationGFP was removed as a BamHI/NotI cassette and replaced with mRFP.
backbone_originClontech
backbone_size3950
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Dominant negative Dynamin 1 mutant tagged with mRFP
origin :
37
pi :
alt_names
DNM1
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5None
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDnm
geneDnm1
id140694
genbank_ids
mutationDynamin-1(K44A)-mRFP was produced from Dynamin-1(K44A)-GFP by replacing GFP with mRFP.
nameDynamin-1(K44A)-mRFP
shRNA_sequence
size3336
species
10116
Rattus norvegicus
32644
Other
Discosoma sp.
tags
locationC terminal on insert
tagmRFP
plasmid copy :
Dyn1(K44A)-GFP was obtained from Pietro De Camilli, Howard Hughes Medical Institute, Yale University, New Haven, CT. mRFP was obtained from Roger Tsien, University of California, San Diego, California.
resistance markers :
2434
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA