pJG/ Inducible ALPHA MHC | Addgene 55792 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pJG/ Inducible ALPHA MHC

catalog :

55792

citations: 1

Reference
Sanbe A, Gulick J, Hanks M, Liang Q, Osinska H, Robbins J. Reengineering inducible cardiac-specific transgenesis with an attenuated myosin heavy chain promoter. Circ Res. 2003;92:609-16 pubmed

product information

Catalog Number :

55792

Product Name :

pJG/ Inducible ALPHA MHC

article :

doi	10.1161/01.RES.0000065442.64694.9F
id	8563
pubmed_id	12623879

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript II SK+

backbone_mutation

backbone_origin

backbone_size

promoter

Inducible ALPHA MHC

sequencing_primer_3

M13R

sequencing_primer_5

M13

vector_types

Mammalian Expression

growth notes :

The distance between the two Not1 sites is ~6kb Clone into the Sal1 site (or Sal/Hind3 site). Release the pBluescript backbone with Not1 for transgenics. This construct consists of a full-length -MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.

growth strain :

transgene expression driven by inducible ALPHA MHC promoter

origin :

resistance markers :

1990

tags :

High Copy