This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
G protein alpha-s-YFP
catalog :
55781
citations: 5
Reference
Buenaventura T, Bitsi S, Laughlin W, Burgoyne T, Lyu Z, Oqua A, et al. Agonist-induced membrane nanodomain clustering drives GLP-1 receptor responses in pancreatic beta cells. PLoS Biol. 2019;17:e3000097 pubmed publisher
Civciristov S, Ellisdon A, Suderman R, Pon C, Evans B, Kleifeld O, et al. Preassembled GPCR signaling complexes mediate distinct cellular responses to ultralow ligand concentrations. Sci Signal. 2018;11: pubmed publisher
Buenaventura T, Kanda N, Douzenis P, Jones B, Bloom S, Chabosseau P, et al. A Targeted RNAi Screen Identifies Endocytic Trafficking Factors That Control GLP-1 Receptor Signaling in Pancreatic β-Cells. Diabetes. 2018;67:385-399 pubmed publisher
Gupta V, Bhandari D, Leyme A, Aznar N, Midde K, Lo I, et al. GIV/Girdin activates Gαi and inhibits Gαs via the same motif. Proc Natl Acad Sci U S A. 2016;113:E5721-30 pubmed publisher
Yost E, Mervine S, Sabo J, Hynes T, Berlot C. Live cell analysis of G protein beta5 complex formation, function, and targeting. Mol Pharmacol. 2007;72:812-25 pubmed
product information
Catalog Number :
55781
Product Name :
G protein alpha-s-YFP
article :
doi10.1124/mol.107.038075
id8544
pubmed_id17596375
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA1/Amp
backbone_mutation
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of z. Effects of palmitoylation and myristoylation on z signaling. J. Biol. Chem. 270, 9667-9675
growth strain :
This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
origin :
37
pi :
alt_names
Gnas
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5HindIII
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALEX, G-alpha-8, Gnas1, Gnpas, Nesp55, SCG6
geneGnas
id24896
genbank_ids
mutationMet was substituted for Gln69 in EYFP (Clontech). BamHI sites in the alpha-s cDNA were removed and alpha-s residues 73-84 were replaced with Gly-Ser adding a unique BamHI site.
namealpha-s-EE YFP
shRNA_sequence
size2303
species
10116
Rattus norvegicus
tags
location
tagEYFP flanked by SGGGS on each side was inserted in place of alpha-s residues 73-84.
location
taginternal EE epitope (residues 189-194 in alpha-s sequence are EYMPTE)
plasmid copy :
Alpha-s-EE was produced in the laboratory of Henry Bourne. EYFP purchased from Clontech.
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA