This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
G protein alpha-o-R179C-EE
catalog :
55780
citations: 1
Reference
Yost E, Mervine S, Sabo J, Hynes T, Berlot C. Live cell analysis of G protein beta5 complex formation, function, and targeting. Mol Pharmacol. 2007;72:812-25 pubmed
product information
Catalog Number :
55780
Product Name :
G protein alpha-o-R179C-EE
article :
doi10.1124/mol.107.038075
id8544
pubmed_id17596375
bacterial resistance :
Ampicillin
cloning :
backbonepcDNAI/Amp
backbone_mutation
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.
origin :
37
pi :
alt_names
Gnao
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGnao, Hg1g, RATBPGTPC
geneGnao1
id50664
genbank_ids
mutationArginine 179 was replaced with cysteine
nameG protein alpha-o with constitutively activating R179C mutation
shRNA_sequence
size1382
species
10116
Rattus norvegicus
tags
location
tagEE epitope was introduced internally with D167E and Q169M mutations.
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA