This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cer(1-158)-beta-1 in pcDNAI/Amp
catalog :
55707
citations: 2
Reference
White A, Jean Alphonse F, Fang F, Pena K, Liu S, König G, et al. Gq/11-dependent regulation of endosomal cAMP generation by parathyroid hormone class B GPCR. Proc Natl Acad Sci U S A. 2020;117:7455-7460 pubmed publisher
Yost E, Mervine S, Sabo J, Hynes T, Berlot C. Live cell analysis of G protein beta5 complex formation, function, and targeting. Mol Pharmacol. 2007;72:812-25 pubmed
product information
Catalog Number :
55707
Product Name :
Cer(1-158)-beta-1 in pcDNAI/Amp
article :
doi10.1124/mol.107.038075
id8544
pubmed_id17596375
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA1/Amp
backbone_mutationBglII introduced to polylinker of pcDNA1/Amp 3' of BamHI site.
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Reference for monomeric Cerulean: Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
growth strain :
An amino-terminal fragment of mCerulean was fused to Gbeta1. When co-expressed with a carboxyl terminal CFP fragment fused to a Ggamma subunit, a fluorescent signal s produced.
origin :
37
pi :
alt_names
transducin beta
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BamHI
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHG2A, MDS, MRD42
geneGNB1
id2782
genbank_ids
mutationGBeta-1 was amplified via PCR, which removed an internal BglII site and added an N-terminal linker sequence (RSIAT), a 5' BamHI site, and a 3' BglII site. Cloning into the BglII site of Cer(1-158) destroyed the BamHI site.
namemCer(1-158)-beta 1
shRNA_sequence
size1483
species
9606
Homo sapiens
32644
Other
Aequorea victoria
tags
locationN terminal on insert
tagCer(1-158) was fused to the amino terminus of Gbeta1.
plasmid copy :
Monomeric Cerulean was obtained from David Piston, Vanderbilt University, Nashville, TN. Beta-1 cDNA obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville PA
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA