This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNATM3.1/V5-His TOPO-PKCi-K274W
catalog :
55690
citations: 2
Reference
Muccioli M, Qaisi D, Herman K, Plageman T. Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition. Dev Biol. 2016;412:32-43 pubmed publisher
Wald F, Oriolo A, Mashukova A, Fregien N, Langshaw A, Salas P. Atypical protein kinase C (iota) activates ezrin in the apical domain of intestinal epithelial cells. J Cell Sci. 2008;121:644-54 pubmed publisher
product information
Catalog Number :
55690
Product Name :
pcDNATM3.1/V5-His TOPO-PKCi-K274W
article :
doi10.1242/jcs.016246
id8543
pubmed_id18270268
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1/V5-His TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size5523
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
K274W is a kinase dead mutation.
growth strain :
expresses dominant negative human PKCi tagged with V5
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDXS1179E, PKCI, nPKC-iota
genePRKCI
id5584
genbank_ids
NM_002740.4
NP_002731.3
mutationA120E and K274W
nameHomo sapiens protein kinase C, iota (PRKCI)
shRNA_sequence
size1790
species
9606
Homo sapiens
tags
locationC terminal on insert
tagV5/His
resistance markers :
1986
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA