This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cer(1-158)-gamma-10 in pcDNAI/Amp
catalog :
55627
citations: 1
Reference
Mervine S, Yost E, Sabo J, Hynes T, Berlot C. Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits. Mol Pharmacol. 2006;70:194-205 pubmed
product information
Catalog Number :
55627
Product Name :
Cer(1-158)-gamma-10 in pcDNAI/Amp
article :
doi10.1124/mol.106.022616
id8487
pubmed_id16641313
bacterial resistance :
Ampicillin
cloning :
backbonepcDNAI/Amp
backbone_mutationA BglII site was introduced into the polylinker of pcDNAI/Amp 3' to the BamHI site.
backbone_originInvitrogen
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Reference for monomeric Cerulean: Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
growth strain :
An N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
origin :
37
pi :
alt_names
GNG10
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BamHI
promoterCMV
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGNG10
id2790
genbank_ids
mutationGgamma-10 was amplified by PCR, which added a BamHI site and a linker sequence (Arg-Ser) to the 5' end and a 3' BglII site. Cloning into the BglII site of Cer(1-158) destroyed the BamHI site.
nameCer(1-158)-gamma-10
shRNA_sequence
size658
species
9606
Homo sapiens
32644
Other
Aequorea victoria
tags
locationN terminal on insert
tagCer(1-158) was fused to the amino terminus of Ggamma-10.
plasmid copy :
Monomeric Cerulean was obtained from David Piston, Vanderbilt University, Nashville, TN. Gamma-10 cDNA was obtained from Janet Robishaw, Weis Center for Research, Geisinger Clinic, Danville, PA.
resistance markers :
2434
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA