CFP(159-238)-beta-1 in pcDNAI/Amp | Addgene 55592 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

CFP(159-238)-beta-1 in pcDNAI/Amp

catalog :

55592

citations: 1

Reference
Mervine S, Yost E, Sabo J, Hynes T, Berlot C. Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits. Mol Pharmacol. 2006;70:194-205 pubmed

product information

Catalog Number :

55592

Product Name :

CFP(159-238)-beta-1 in pcDNAI/Amp

article :

doi	10.1124/mol.106.022616
id	8487
pubmed_id	16641313

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNAI/Amp

backbone_mutation

A BglII site was introduced into the polylinker of pcDNA1/Amp 3' to the BamHI site.

backbone_origin

Invitrogen

backbone_size

4800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

origin :

pi :

alt_names

transducin beta

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HG2A, MDS, MRD42
gene	GNB1
id	2782

genbank_ids

mutation

CFP(159-238) includes a substitution of His for Asn-165. GBeta-1 was amplified via PCR, which removed an internal BglII site and added an N-terminal linker sequence (RSIAT), a 5' BamHI site, and a 3' BglII site. Cloning into the BglII site of CFP(159-238) destroyed the BamHI site.

name

CFP (159-238)-Beta 1

shRNA_sequence

size

1258

species

9606

Homo sapiens

32644

Other

Aequorea victoria