YFP(159-238)-gamma-7 in pcDNAI/Amp | Addgene 54473 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

YFP(159-238)-gamma-7 in pcDNAI/Amp

catalog :

54473

citations: 1

Reference
Hynes T, Tang L, Mervine S, Sabo J, Yost E, Devreotes P, et al. Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. J Biol Chem. 2004;279:30279-86 pubmed

product information

Catalog Number :

54473

Product Name :

YFP(159-238)-gamma-7 in pcDNAI/Amp

article :

doi	10.1074/jbc.M401432200
id	8491
pubmed_id	15136579

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNAI/Amp

backbone_mutation

A BglII site was introduced to into the polylinker of pcDNAI/Amp 3' to the BamHI site.

backbone_origin

Invitrogen

backbone_size

4800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

A carboxyl-terminal YFP fragment was fused to Ggamma-7. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

origin :

pi :

alt_names

GNG7

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	GNG7
id	2788

genbank_ids

mutation

Ggamma-7 was amplified by PCR, which added a BamHI site and a linker sequence (Arg-Ser) to the 5' end and a 3' BglII site. Cloning into the BglII site of YFP(159-238) destroyed the BamHI site.

name

YFP(159-238)-gamma-7

shRNA_sequence

size

433

species

9606

Homo sapiens

32644

Other

Aequorea victoria