YFP(1-158)-beta-1 in pcDNAI/Amp | Addgene 54468 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

YFP(1-158)-beta-1 in pcDNAI/Amp

catalog :

54468

citations: 1

Reference
Hynes T, Tang L, Mervine S, Sabo J, Yost E, Devreotes P, et al. Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. J Biol Chem. 2004;279:30279-86 pubmed

product information

Catalog Number :

54468

Product Name :

YFP(1-158)-beta-1 in pcDNAI/Amp

article :

doi	10.1074/jbc.M401432200
id	8491
pubmed_id	15136579

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNAI/Amp

backbone_mutation

BglII introduced to polylinker of pcDNA1/amp 3' of BamHI site.

backbone_origin

Invitrogen

backbone_size

4800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

origin :

pi :

alt_names

transducin beta

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HG2A, MDS, MRD42
gene	GNB1
id	2782

genbank_ids

mutation

GBeta-1 was amplified via PCR, which removed an internal BglII site and added an N-terminal linker sequence (RSIAT), a 5' BamHI site, and a 3' BglII site. Cloning into the BglII site of YFP(1-158) destroyed the BamHI site. PCR amplification of YFP (1-158) introduced a substitution of M for Q69.

name

YFP (1-158)/beta-1

shRNA_sequence

size

1483

species

9606

Homo sapiens

32644

Other

Aequorea victoria