hTRIM65_EGFP_N1 | Addgene 54320 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

hTRIM65_EGFP_N1

catalog :

54320

citations: 1

Reference
Li S, Wang L, Fu B, Berman M, Diallo A, Dorf M. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014;111:6970-5 pubmed publisher

product information

Catalog Number :

54320

Product Name :

hTRIM65_EGFP_N1

article :

doi	10.1073/pnas.1322545111
id	8429
pubmed_id	24778252

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-N1

backbone_mutation

human TRIM65 in inserted into pEGFP-N1 with 5': XhoI/SalI insertion + kozak initiation sequence, and 3': EcoRI insertion. The stop codon is removed, and one addidional C nucleotide is added before the EcoRI site to maintain the reading frame. The construct contains : (partial XhoI site) CTCGACGCCACC-human TRIM65 CDS(1551bp, no stop)-C-(GAATTC EcoRI site)-44bp-EGFP.

backbone_origin

Clontech

backbone_size

4723

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Human TRIM65 cDNA was cloned from the T98 glioblastoma cell line (ATCC). The CDS (coding sequence) is identical to Genbank BC021259.2.

growth strain :

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

origin :

pi :

alt_names

tripartite motif containing 65

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	XhoI
promoter	CMV
sequencing_primer_3	EGFP reverse (tccagctcgaccaggatg)
sequencing_primer_5	CMV forward (cgtgtacggtgggaggtct)
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	4732463G12Rik
gene	TRIM65
id	201292

genbank_ids

BC021259.2 (has same CDS sequence)

mutation

name

TRIM65

shRNA_sequence

size

1558

species

9606

Homo sapiens