This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMVhTRIM65_3Flag
catalog :
53966
citations: 1
Reference
Li S, Wang L, Fu B, Berman M, Diallo A, Dorf M. TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014;111:6970-5 pubmed publisher
product information
Catalog Number :
53966
Product Name :
pCMVhTRIM65_3Flag
article :
doi10.1073/pnas.1322545111
id8429
pubmed_id24778252
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-3Tag-8
backbone_mutationContruct: [XhoI site]CTCGAG-CTG-[HindIII]AAGCTT-(Kozak seq]GCC-insert[hTRIM65 CDS without stop, 1551bp]-[Clai]ATCGAT. The commercial multiple cloning site has been replaced as indicated. Backbone's 3xFLAGs are in frame with the restriction sites.
backbone_originAgilent (formerly Stratagene)
backbone_size5171
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cloned from human T98 glioblastoma line. The coding sequence is identical to that in BC021259.2
growth strain :
Human TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus.
origin :
37
pi :
alt_names
tripartite motif containing 65
cloning
clone_methodRestriction Enzyme
cloning_site_3Clai
cloning_site_5HindIII
promoterCMV
sequencing_primer_3T7, BGH reverse
sequencing_primer_5CMV, T3 (28bp from insert)
site_3_destroyed
site_5_destroyed
entrez_gene
aliases4732463G12Rik
geneTRIM65
id201292
genbank_ids
BC021259.2 has same CDS.
mutation
nameTRIM65
shRNA_sequence
size1554
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag3x FLAG
resistance markers :
120
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA