This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS-H2B-citrine
catalog :
53752
citations: 1
Reference
Megason S. In toto imaging of embryogenesis with confocal time-lapse microscopy. Methods Mol Biol. 2009;546:317-32 pubmed publisher
product information
Catalog Number :
53752
Product Name :
pCS-H2B-citrine
article :
doi10.1007/978-1-60327-977-2_19
id8404
pubmed_id19378112
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_originRZPD
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid was created using the QuikChange protocol to perform site directed mutagenesis to make Q69M mutation to convert EYFP of pCS-H2B-EYFP (Addgene plasmid # 53746) into citrine. Use NotI/SP6 for transcription.
growth strain :
Contains fusion of human Histone H2B to citrine.
origin :
37
pi :
alt_names
histone cluster 2, H2be
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promotersimian CMV IE94
sequencing_primer_3EBV-rev
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
geneH2BC21
id8349
genbank_ids
mutation
namehistone H2B
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagcitrine
plasmid copy :
H2B was obtained from Dr. Reinhard Koster.
resistance markers :
1968
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA