pCS-H2B-cerulean | Addgene 53748 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCS-H2B-cerulean

catalog :

53748

citations: 2

Reference
Madigan C, Cambier C, Kelly Scumpia K, Scumpia P, Cheng T, Zailaa J, et al. A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy. Cell. 2017;170:973-985.e10 pubmed publisher
Megason S. In toto imaging of embryogenesis with confocal time-lapse microscopy. Methods Mol Biol. 2009;546:317-32 pubmed publisher

product information

Catalog Number :

53748

Product Name :

pCS-H2B-cerulean

article :

doi	10.1007/978-1-60327-977-2_19
id	8404
pubmed_id	19378112

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2+

backbone_mutation

backbone_origin

RZPD

backbone_size

4095

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Use NotI/SP6 for sense RNA. This plasmid was made using a triple ligation of the H2B from XhoI/AgeI of pCS-H2B-EGFP (Addgene plasmid 53744), the cerulean from AgeI/BsrG1 of pCeruleanC1 and pCS from BsrG1/XhoI of pCS-H2B-EGFP. This puts H2B-cerulean in the SnaBI site of pCS.

growth strain :

Contains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.

origin :

pi :

alt_names

histone cluster 2, H2be

cloning

clone_method	Restriction Enzyme
cloning_site_3	SnaBI
cloning_site_5	SnaBI
promoter	simian CMV IE94
sequencing_primer_3	EBV-rev
sequencing_primer_5	SP6
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
gene	H2BC21
id	8349

genbank_ids

mutation

name

histone H2B

shRNA_sequence

size

species

9606

Homo sapiens