This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS-H2B-mRFP1
catalog :
53745
citations: 8
Reference
Tsuda N, Fukagawa R, Sueda S. Does the nuclear envelope retain its identity during mitosis?. FEBS Lett. 2022;: pubmed publisher
Moreno Marmol T, Ledesma Terrón M, Tabanera N, Martín Bermejo M, Cardozo M, Cavodeassi F, et al. Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis. elife. 2021;10: pubmed publisher
Schuck P, Ball L, Stewart J. The DNA-binding protein CST associates with the cohesin complex and promotes chromosome cohesion. J Biol Chem. 2021;:101026 pubmed publisher
Lane M, Mis E, Khokha M. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harb Protoc. 2021;: pubmed publisher
Zenker J, White M, Gasnier M, Alvarez Y, Lim H, Bissiere S, et al. Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation. Cell. 2018;173:776-791.e17 pubmed publisher
Vázquez Diez C, FitzHarris G. Correlative Live Imaging and Immunofluorescence for Analysis of Chromosome Segregation in Mouse Preimplantation Embryos. Methods Mol Biol. 2018;1769:319-335 pubmed publisher
Zhao Z, White M, Alvarez Y, Zenker J, Bissiere S, Plachta N. Quantifying transcription factor-DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy. Nat Protoc. 2017;12:1458-1471 pubmed publisher
Megason S. In toto imaging of embryogenesis with confocal time-lapse microscopy. Methods Mol Biol. 2009;546:317-32 pubmed publisher
product information
Catalog Number :
53745
Product Name :
pCS-H2B-mRFP1
article :
doi10.1007/978-1-60327-977-2_19
id8404
pubmed_id19378112
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_originRZPD
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cut with NotI and use SP6 for sense RNA. Created by replacing EGFP in in pCS-H2B-EGFP (Addgene plasmid 53744) with mRFP using AgeI and SnaBI.
growth strain :
Contains a fusion of human histone H2B to monomeric red fluorescent protein 1 in the pCS2+ expression vector
origin :
37
pi :
alt_names
histone cluster 2, H2be
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5unknown
promotersimian CMV IE94
sequencing_primer_3EBV-rev
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
geneH2BC21
id8349
genbank_ids
mutation
namehistone H2B
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmRFP
plasmid copy :
H2B was obtained from Dr. Reinhard Koster.
resistance markers :
1968
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA