This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS-H2B-EGFP
catalog :
53744
citations: 5
Reference
Sousa Ortega A, V xe1 zquez Mar xed n J, Sanabria Reinoso E, Corbacho J, Polvillo R, Campoy L xf3 pez A, et al. A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly. Nat Commun. 2023;14:2804 pubmed publisher
Lane M, Mis E, Khokha M. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harb Protoc. 2021;: pubmed publisher
Sepúlveda Ramírez S, Toledo Jacobo L, Garno C, Pal D, Ross C, Ellis A, et al. Live-cell fluorescence imaging of echinoderm embryos. Methods Cell Biol. 2019;151:379-397 pubmed publisher
Sequerra E, Goyal R, Castro P, Levin J, Borodinsky L. NMDA Receptor Signaling Is Important for Neural Tube Formation and for Preventing Antiepileptic Drug-Induced Neural Tube Defects. J Neurosci. 2018;38:4762-4773 pubmed publisher
Megason S. In toto imaging of embryogenesis with confocal time-lapse microscopy. Methods Mol Biol. 2009;546:317-32 pubmed publisher
product information
Catalog Number :
53744
Product Name :
pCS-H2B-EGFP
article :
doi10.1007/978-1-60327-977-2_19
id8404
pubmed_id19378112
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_originRZPD
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cut with NotI and use SP6 for sense RNA.
growth strain :
Contains human Histone H2B fused to EGFP in the pCS2+ expression vector
origin :
37
pi :
alt_names
histone cluster 2, H2be
cloning
clone_methodRestriction Enzyme
cloning_site_3SnaB1
cloning_site_5SnaB1
promotersimian CMV IE94
sequencing_primer_3EBV-rev
sequencing_primer_5SP6
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesGL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
geneH2BC21
id8349
genbank_ids
mutation
namehistone H2B
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
plasmid copy :
H2B was obtained from Dr. Reinhard Koster.
resistance markers :
1968
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA