This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3/Flag-WTAP
catalog :
53741
citations: 5
Reference
Cui X, Nilsson K, Kajitani N, Schwartz S. Overexpression of m6A-factors METTL3, ALKBH5, and YTHDC1 alters HPV16 mRNA splicing. Virus Genes. 2022;58:98-112 pubmed publisher
Han D, Longhini A, Zhang X, Hoang V, Wilson M, Kosik K. Dynamic assembly of the mRNA m6A methyltransferase complex is regulated by METTL3 phase separation. PLoS Biol. 2022;20:e3001535 pubmed publisher
He R, Jiang J, Hu X, Lei M, Li J, Luo W, et al. Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression. Cancer Cell Int. 2021;21:616 pubmed publisher
Sun H, Zhu A, Gao Y, Terajima H, Fei Q, Liu S, et al. Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation. Mol Cell. 2020;80:633-647.e7 pubmed publisher
Liu J, Yue Y, Han D, Wang X, Fu Y, Zhang L, et al. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat Chem Biol. 2014;10:93-5 pubmed publisher
product information
Catalog Number :
53741
Product Name :
pcDNA3/Flag-WTAP
article :
doi10.1038/nchembio.1432
id8402
pubmed_id24316715
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutationN.A.
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
For expression of WTAP (Wilms tumor 1 associated protein) in human cells
origin :
37
pi :
alt_names
WTAP
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterT7
sequencing_primer_35' ATTTAGGTGACACTATAG 3'
sequencing_primer_55' TAATACGACTCACTATAGGG 3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMum2
geneWTAP
id9589
genbank_ids
NM_001270531.1
mutation
nameWilms tumor 1 associated protein
shRNA_sequence
size1191
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG tag
plasmid copy :
cDNA was obtained from Open Biosystems
resistance markers :
1886
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA