This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
STK3A-c042
catalog :
53622
product information
Catalog Number :
53622
Product Name :
STK3A-c042
article :
doi
id8386
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepNIC28-Bsa4
backbone_mutation
backbone_originSGC
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
T7/lac regulated, N-terminal His-tag, TEV, LIC cloning using BsaI cleavage/T4 polymerase, SacB stuffer fragment, pET28 backbone. http://www.thesgc.org/structures/4L0N/ Use BL21(DE3)-R3-pRARE2 strain for expression.
growth strain :
bacterial expression plasmid
inserts :
BL21(DE3)-R3-pRARE2
origin :
37
pi :
alt_names
4L0N
gi 5454094
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKRS1, MST2
geneSTK3
id6788
genbank_ids
mutationBacterial expression for structure determination; may not be full ORF, see comments
nameSTK3A
shRNA_sequence
size244
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHis-6-TEV
resistance markers :
1369
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA