This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Zebrafish ArcLight Q175
catalog :
53616
citations: 1
Reference
Han Z, Jin L, Platisa J, Cohen L, Baker B, Pieribone V. Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics. PLoS ONE. 2013;8:e81295 pubmed publisher
product information
Catalog Number :
53616
Product Name :
Zebrafish ArcLight Q175
article :
doi10.1371/journal.pone.0081295
id8367
pubmed_id24312287
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size5701
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Genetically encoded voltage sensor ArcLight with improved kinetics
origin :
37
pi :
alt_names
Zebrafish-Q175
Dr-VSD ArcLight Q175
cloning
clone_methodRestriction Enzyme
cloning_site_3BgIII
cloning_site_5HindIII
promoterCMV
sequencing_primer_3ACCTTCGGGCATGGCACTCTTG
sequencing_primer_5AGAGGGTGAAGGTGATGCAACATAC
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasestpip, vsp, wu:fd20e11, wu:fi24b06
genetpte
id327292
genbank_ids
NP_001020629.1
AY533296
mutationDr-VSD contains R153Q mutation and an amino acid E (GAG) introduced immediately after starting M (ATG); Dr-VSP is truncated at Q176 (Q175 in the original Dr-VSP sequence) and super ecliptic pHluorin containing a A227D (FP numbering) mutation is fused to the C-terminal (after Q176).
nameZebrafish ArcLight-Q175
shRNA_sequence
size1254
species
7955
Danio rerio
100
Synthetic
32644
Other
Aequorea victoria
tags
resistance markers :
1258
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA