This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNatB (pACYCduet-naa20-naa25)
catalog :
53613
citations: 13
Reference
Zhang S, Dauer K, Stroh xe4 ker T, Tatenhorst L, Caldi Gomes L, Mayer S, et al. Alpha-synuclein fibrils amplified from multiple system atrophy and Parkinson's disease patient brain spread after intracerebral injection into mouse brain. Brain Pathol. 2023;: pubmed publisher
Tang Q, Pollard L, Homa K, Kovar D, Trybus K. Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins. Cytoskeleton (Hoboken). 2023;80:77-92 pubmed publisher
Yang X, Wang B, Hoop C, Williams J, Baum J. NMR unveils an N-terminal interaction interface on acetylated-α-synuclein monomers for recruitment to fibrils. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Mohapatra A, Chaudhary N. N-terminal acetylation does not alter α-synuclein's interfacial properties. Int J Biol Macromol. 2021;174:69-76 pubmed publisher
Curry A, Fernández R, Pagani T, Abeyawardhane D, Trahan M, Lucas H. Mapping of Photochemically-Derived Dityrosine across Fe-Bound N-Acetylated α-Synuclein. Life (Basel). 2020;10: pubmed publisher
Strohäker T, Jung B, Liou S, Fernandez C, Riedel D, Becker S, et al. Structural heterogeneity of α-synuclein fibrils amplified from patient brain extracts. Nat Commun. 2019;10:5535 pubmed publisher
Yang X, Williams J, Yan R, Mouradian M, Baum J. Increased Dynamics of α-Synuclein Fibrils by β-Synuclein Leads to Reduced Seeding and Cytotoxicity. Sci Rep. 2019;9:17579 pubmed publisher
Wördehoff M, Hoyer W. α-Synuclein Aggregation Monitored by Thioflavin T Fluorescence Assay. Bio Protoc. 2018;8: pubmed publisher
Fernández R, Lucas H. Isolation of recombinant tetrameric N-acetylated α-synuclein. Protein Expr Purif. 2018;152:146-154 pubmed publisher
Rovere M, Powers A, Patel D, Bartels T. pTSara-NatB, an improved N-terminal acetylation system for recombinant protein expression in E. coli. PLoS ONE. 2018;13:e0198715 pubmed publisher
Young T, Wedd A, Xiao Z. Evaluation of Cu(i) binding to the E2 domain of the amyloid precursor protein - a lesson in quantification of metal binding to proteins via ligand competition. Metallomics. 2018;10:108-119 pubmed publisher
Wördehoff M, Shaykhalishahi H, Groß L, Gremer L, Stoldt M, Buell A, et al. Opposed Effects of Dityrosine Formation in Soluble and Aggregated α-Synuclein on Fibril Growth. J Mol Biol. 2017;429:3018-3030 pubmed publisher
Johnson M, Coulton A, Geeves M, Mulvihill D. Targeted amino-terminal acetylation of recombinant proteins in E. coli. PLoS ONE. 2010;5:e15801 pubmed publisher
product information
Catalog Number :
53613
Product Name :
pNatB (pACYCduet-naa20-naa25)
article :
doi10.1371/journal.pone.0015801
id8384
pubmed_id21203426
bacterial resistance :
Chloramphenicol
cloning :
backbonepACYCduet
backbone_mutation
backbone_originnovagen
backbone_size4008
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This plasmid contains an A2520G (Asp to Gly) mutation within naa25 insert. The depositor states that this mutation does not affect plasmid function.
growth strain :
Allows expression of the fission yeast NatB complex - chloramphenicol marker
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5Nde1
promoterT7
sequencing_primer_3T7 Terminator Primer
sequencing_primer_5DuetUP2 Primer
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesSPCC16C4.12, nat3
genenaa20
id2539279
genbank_ids
mutation
namenaa20
shRNA_sequence
size543
species
4896
Schizosaccharomyces pombe
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal1
cloning_site_5Sal1
promoterT7
sequencing_primer_3DuetDOWN1 Primer
sequencing_primer_5ACYCDuetUP1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPBC1215.02c, arm1, mdm20
genenaa25
id2540082
genbank_ids
mutation
namenaa25
shRNA_sequence
size2436
species
4896
Schizosaccharomyces pombe
tags
resistance markers :
1963
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA