pHRIG-Akt3 | Addgene 53596 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHRIG-Akt3

catalog :

53596

citations: 2

Reference
Nowak D, Cho H, Herzka T, Watrud K, DeMarco D, Wang V, et al. MYC Drives Pten/Trp53-Deficient Proliferation and Metastasis due to IL6 Secretion and AKT Suppression via PHLPP2. Cancer Discov. 2015;5:636-51 pubmed publisher
Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky R, et al. Akt isoforms differentially protect against stroke-induced neuronal injury by regulating mTOR activities. J Cereb Blood Flow Metab. 2013;33:1875-85 pubmed publisher

product information

Catalog Number :

53596

Product Name :

pHRIG-Akt3

article :

doi	10.1038/jcbfm.2013.132
id	8371
pubmed_id	23942361

bacterial resistance :

Ampicillin

cloning :

backbone

pHR-IG (pHR tripCMV-IRES-eGFP)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

cAkt3-HA (pcDNA3.1) provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University. cAkt3 was fused with a Gag polypeptide and lacks the pleckstrin homology (PH) domain; the expression of a myristoylation signal enables Akt3 protein to attach to the cell membrane and become constitutively phosphorylated, thus activated (Aimee 1996).

growth strain :

lentiviral expression of constitutively active human Akt3 and EGFP

origin :

pi :

alt_names

Akt3

v-akt murine thymoma viral oncogene homolog 3

cloning

clone_method	Restriction Enzyme
cloning_site_3	SacII
cloning_site_5	SacII
promoter	CMV
sequencing_primer_3	pCDH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
gene	AKT3
id	10000

genbank_ids

mutation

constitutively active (myristoylated version)

name

cAkt3

shRNA_sequence

size

species

9606

Homo sapiens