This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAtLCYbSK
catalog :
53588
citations: 1
| Reference |
|---|
Cunningham F, Lee H, Gantt E. Carotenoid biosynthesis in the primitive red alga Cyanidioschyzon merolae. Eukaryot Cell. 2007;6:533-45 pubmed
|
product information
Catalog Number :
53588
Product Name :
pAtLCYbSK
article :
| doi | 10.1128/EC.00265-06 |
| id | 8301 |
| pubmed_id | 17085635 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBluescript SK- | |
| backbone_mutation | The entire open reading frame of an Arabidopsis lcyB cDNA [GenBank U50739.1] in plasmid pZL1 (the original cDNA library plasmid) was excised as an EcoRI-HindIII fragment of ca. 1.8 kB and cloned in the same sites of pBluescript SK- so that the cDNA was fused, in frame, to the N terminal portion of the lacZ gene and under the control of the lac promoter. | |
| backbone_origin | Stratagene | |
| backbone_size | 2958 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth strain :
Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.
growth temp :
Can be used with pAC-LYC or pAC-LYCipi to produce beta-carotene in E. coli strain TOP10. For this purpose, grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness, or grow on agar plates at room temperature for 3-7 days.
origin :
37
pi :
|
resistance markers :
| 1973 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments