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product summary
company name :
Addgene
product type :
cDNA
product name :
y2
catalog :
53586
citations: 1
product information
Catalog Number :
53586
Product Name :
y2
article :
| doi | 10.1105/tpc.8.9.1613 |
| id | 8297 |
| pubmed_id | 8837512 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBluescript SK- |
| backbone_mutation | This is an Arabidopsis thaliana cDNA library plasmid that contains an lcyE cDNA that encodes a lycopene epsilon-cyclase. The cDNA is in the reverse orientation relative to the lac promoter. |
| backbone_origin | Stratagene |
| backbone_size | 2958 |
| promoter | |
| sequencing_primer_3 | |
| sequencing_primer_5 | |
| vector_types |
growth strain :
Produces an active Arabidopsis thaliana lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to make delta-carotene in E. coli.
growth temp :
This plasmid produces an active lycopene epsilon-monocyclase in E. coli, and can be used with pAC-LYC or pAC-LYCipi in E. coli strain TOP10 (most other E. coli strains are also suitable) to produce delta-carotene. In this case, grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness, or grow on agar plates at room temperature for 3-7 days.
origin :
37
pi :
|
resistance markers :
| 1973 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments