pHRIG-Akt1 | Addgene 53583 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHRIG-Akt1

catalog :

53583

citations: 10

Reference
Zhang K, Burns C, Skinner M, Lombard D, Miller R, Endicott S. PTEN is both an activator and a substrate of chaperone-mediated autophagy. J Cell Biol. 2023;222: pubmed publisher
Dehghanian S, Pan C, Lee J, Shiue Y. ABT-751 Induces Multiple Anticancer Effects in Urinary Bladder Urothelial Carcinoma-Derived Cells: Highlighting the Induction of Cytostasis through the Inhibition of SKP2 at Both Transcriptional and Post-Translational Levels. Int J Mol Sci. 2021;22: pubmed publisher
Tan H, Hu B, Xie F, Zhu C, Cheng Z. Anisomycin sensitizes non-small-cell lung cancer cells to chemotherapeutic agents and epidermal growth factor receptor inhibitor via suppressing PI3K/Akt/mTOR. Fundam Clin Pharmacol. 2020;: pubmed publisher
Yang P, Xie J, Li Y, Lin H, Fenske W, Clementino M, et al. Deubiquitinase USP7-mediated MCL-1 up-regulation enhances Arsenic and Benzo(a)pyrene co-exposure-induced Cancer Stem Cell-like property and Tumorigenesis. Theranostics. 2020;10:9050-9065 pubmed publisher
Bruyère J, Abada Y, Vitet H, Fontaine G, Deloulme J, Ces A, et al. Presynaptic APP levels and synaptic homeostasis are regulated by Akt phosphorylation of huntingtin. elife. 2020;9: pubmed publisher
Wu W, Lin J, Pan C, Chan T, Liu C, Wu W, et al. Amplification-driven BCL6-suppressed cytostasis is mediated by transrepression of FOXO3 and post-translational modifications of FOXO3 in urinary bladder urothelial carcinoma. Theranostics. 2020;10:707-724 pubmed publisher
Wei R, Wu W, Pan C, Yu C, Li C, Chen L, et al. Inhibition of the formation of autophagosome but not autolysosome augments ABT-751-induced apoptosis in TP53-deficient Hep-3B cells. J Cell Physiol. 2019;234:9551-9563 pubmed publisher
Li C, Wu W, Chan T, Wang Y, Chen L, Wu W, et al. Transmembrane and Coiled-Coil Domain 1 Impairs the AKT Signaling Pathway in Urinary Bladder Urothelial Carcinoma: A Characterization of a Tumor Suppressor. Clin Cancer Res. 2017;23:7650-7663 pubmed publisher
Nowak D, Cho H, Herzka T, Watrud K, DeMarco D, Wang V, et al. MYC Drives Pten/Trp53-Deficient Proliferation and Metastasis due to IL6 Secretion and AKT Suppression via PHLPP2. Cancer Discov. 2015;5:636-51 pubmed publisher
Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky R, et al. Akt isoforms differentially protect against stroke-induced neuronal injury by regulating mTOR activities. J Cereb Blood Flow Metab. 2013;33:1875-85 pubmed publisher

product information

Catalog Number :

53583

Product Name :

pHRIG-Akt1

article :

doi	10.1038/jcbfm.2013.132
id	8371
pubmed_id	23942361

bacterial resistance :

Ampicillin

cloning :

backbone

pHR-IG (pHR tripCMV-IRES-eGFP)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

cAkt1-HA (pcDNA3.1) provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University. cAkt1 was fused with a Gag polypeptide and lacks the pleckstrin homology (PH) domain; the expression of a myristoylation signal enables Akt1 protein to attach to the cell membrane and become constitutively phosphorylated, thus activated (Aimee 1996).

growth strain :

lentiviral expression of constitutively active human Akt1 and EGFP

origin :

pi :

alt_names

Akt1

thymoma viral proto-oncogene 1

cloning

clone_method	Restriction Enzyme
cloning_site_3	SacII
cloning_site_5	SalI
promoter	CMV
sequencing_primer_3	pCDH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
gene	AKT1
id	207

genbank_ids

mutation

constitutively active (myristoylated version)

name

cAkt1

shRNA_sequence

size

species

9606

Homo sapiens