This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
shRNA Caspase 1 (pSicoR mCherry)
catalog :
53575
citations: 2
Reference
Adams A, Weinman S, Wozniak A. Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILP. Biochem Biophys Res Commun. 2018;503:2619-2624 pubmed publisher
Doitsh G, Galloway N, Geng X, Yang Z, Monroe K, Zepeda O, et al. Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 infection. Nature. 2014;505:509-14 pubmed publisher
product information
Catalog Number :
53575
Product Name :
shRNA Caspase 1 (pSicoR mCherry)
article :
doi10.1038/nature12940
id8369
pubmed_id24356306
bacterial resistance :
Ampicillin
cloning :
backbonepSicoR EF1a-mCherry
backbone_mutationshRNA Caspase 1 inserted
backbone_originpSicoR-Ef1a-mCh was a gift from Bruce Conklin (Addgene plasmid # 31847)
backbone_size7484
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
The shRNA was cloned into the HpaI and XhoI restriction sites as described for pSicoR.
growth strain :
shRNA against human pro-caspase 1
origin :
30
pi :
alt_names
shRNA Caspase 1 mCherry
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5Hpa1
promoterU6 for shRNA; EF1a for mCherry
sequencing_primer_3WPRE-R
sequencing_primer_5TGCAGGGGAAAGAATAGTAGAC
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesICE, IL1BC, P45
geneCASP1
id834
genbank_ids
mutation
nameshRNA Caspase 1
shRNA_sequenceacacgtcttgctctcatta
size
species
9606
Homo sapiens
tags
resistance markers :
176
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA