pET28a(+) 6xHis-TEV-IFIT1 | Addgene 53557 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET28a(+) 6xHis-TEV-IFIT1

catalog :

53557

citations: 3

Reference
Dobieżyńska A, Miszkiewicz J, Darzynkiewicz Z, Tyras M, Stankiewicz Drogon A, Trylska J, et al. Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies. Biochem Biophys Res Commun. 2020;533:391-396 pubmed publisher
Miedziak B, Dobieżyńska A, Darzynkiewicz Z, Bartkowska J, Miszkiewicz J, Kowalska J, et al. Kinetic analysis of IFIT1 and IFIT5 interactions with different native and engineered RNAs and its consequences for designing mRNA-based therapeutics. RNA. 2020;26:58-68 pubmed publisher
Katibah G, Lee H, Huizar J, Vogan J, Alber T, Collins K. tRNA binding, structure, and localization of the human interferon-induced protein IFIT5. Mol Cell. 2013;49:743-50 pubmed publisher

product information

Catalog Number :

53557

Product Name :

pET28a(+) 6xHis-TEV-IFIT1

article :

doi	10.1016/j.molcel.2012.12.015
id	8365
pubmed_id	23317505

bacterial resistance :

Kanamycin

cloning :

backbone

pET28a(+)

backbone_mutation

backbone_origin

Novagen

backbone_size

5369

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

bacterial expression of IFIT1

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NheI
promoter	T7
sequencing_primer_3	T7 terminal
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C56, G10P1, IFI-56, IFI-56K, IFI56, IFIT-1, IFNAI1, ISG56, P56, RNM561
gene	IFIT1
id	3434

genbank_ids

mutation

name

IFIT1

shRNA_sequence

size

species

9606

Homo sapiens