This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-Gm1
catalog :
53528
citations: 1
Reference
Igloi G, Schiefermayr E. Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural specificity variants. FEBS J. 2009;276:1307-18 pubmed publisher
product information
Catalog Number :
53528
Product Name :
pET-Gm1
article :
doi10.1111/j.1742-4658.2009.06866.x
id8352
pubmed_id19187230
bacterial resistance :
Ampicillin
cloning :
backbonepET32a+
backbone_mutation
backbone_originNovagen
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses a plant arginyl-tRNA synthetase in E.coli
growth temp :
For induction, grow at 23 C. Expression results largely in insoluble protein with a low yield of soluble, active enzyme
origin :
37
pi :
alt_names
RARS
cloning
clone_methodRestriction Enzyme
cloning_site_3NcoI
cloning_site_5NcoI
promoterT7
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
FM209045
mutation
namearginyl-tRNA synthetase
shRNA_sequence
size1794
species
32644
Other
Glycin max (soybean)
tags
locationN terminal on backbone
tagThioredoxin
locationN terminal on backbone
tagHis Tag
resistance markers :
1967
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA