This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HPSE-bio-His
catalog :
53407
citations: 2
Reference
Nguyen L, McCord K, Bui D, Bouwman K, Kitova E, Elaish M, et al. Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2. Nat Chem Biol. 2022;18:81-90 pubmed publisher
Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts M, Wright G. A Human Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit α (FcεR1α) as an Activating Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand. Mol Cell Proteomics. 2015;14:1265-74 pubmed publisher
product information
Catalog Number :
53407
Product Name :
HPSE-bio-His
article :
doi10.1074/mcp.M114.046946
id9831
pubmed_id25713122
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutationRat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
backbone_originYves Durocher, NRC
backbone_size6618
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Article ref 125
growth strain :
Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
origin :
37
pi :
alt_names
Heparanase precursor
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHPA, HPA1, HPR1, HPSE1, HSE1
geneHPSE
id10855
genbank_ids
mutation
nameHPSE
shRNA_sequence
size1635
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagrat CD4 d3+4
locationC terminal on backbone
tagenzymatic biotinylation sequence
locationC terminal on backbone
tagHis tag
plasmid copy :
Insert was chemically synthesised.
resistance markers :
919
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA