pCB268 | Addgene 53363 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCB268

catalog :

53363

citations: 3

Reference
Shi J, Hu X, Guo Y, Wang L, Ji J, Li J, et al. A technique for delineating the unfolding requirements for substrate entry into retrotranslocons during endoplasmic reticulum-associated degradation. J Biol Chem. 2019;: pubmed publisher
Weaver B, Weaver Y, Mitani S, Han M. Coupled Caspase and N-End Rule Ligase Activities Allow Recognition and Degradation of Pluripotency Factor LIN-28 during Non-Apoptotic Development. Dev Cell. 2017;41:665-673.e6 pubmed publisher
Brower C, Piatkov K, Varshavsky A. Neurodegeneration-associated protein fragments as short-lived substrates of the N-end rule pathway. Mol Cell. 2013;50:161-71 pubmed publisher

product information

Catalog Number :

53363

Product Name :

pCB268

article :

doi	10.1016/j.molcel.2013.02.009
id	8317
pubmed_id	23499006

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.0 - Nco

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

pcDNA3.0-based plasmid encoding fDHFR-UbK48R-A 42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	n/a
cloning_site_5	HindIII
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	8430436I03Rik
gene	Dhfr
id	13361

genbank_ids

mutation

name

DHFR

shRNA_sequence

size

species

10090

Mus musculus